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Parthenolide-induced apoptosis, autophagy and suppression of proliferation in HepG2 cells.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2014; Vol. 15 (12), pp. 4897-902. - Publication Year :
- 2014
-
Abstract
- Purpose: To investigate the anticancer effects and underlying mechanisms of parthenolide on HepG2 human hepatocellular carcinoma cells.<br />Materials and Methods: Cell viability was assessed by MTT assay and cell apoptosis through DAPI, TUNEL staining and Western blotting. Monodansylcadaverin(MDC) and AO staining were used to detect cell autophagy. Cell proliferation was assessed by Ki67 immunofluorescence staining.<br />Results: Parthenolide induced growth inhibition in HepG2 cells. DAPI and TUNEL staining showed that parthenolide could increase the number of apoptotic nuclei, while reducing the expression of the anti-apoptotic protein Bcl-2 and elevating the expression of related proteins, like p53, Bax, cleaved caspase9 and cleaved caspase3. Parthenolide could induce autophagy in HepG2 cells and inhibited the expression of proliferation-related gene, Ki-67.<br />Conclusions: Parthenolide can exert anti-cancer effects by inducing cell apoptosis, activating autophagy and inhibiting cell proliferation.
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Blotting, Western
Carcinoma, Hepatocellular metabolism
Fluorescent Antibody Technique
Hep G2 Cells
Humans
Liver Neoplasms drug therapy
Liver Neoplasms metabolism
Liver Neoplasms pathology
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Apoptosis drug effects
Autophagy drug effects
Carcinoma, Hepatocellular drug therapy
Carcinoma, Hepatocellular pathology
Cell Proliferation drug effects
Sesquiterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 15
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 24998560
- Full Text :
- https://doi.org/10.7314/apjcp.2014.15.12.4897