Changes in gene expression profile in two multidrug resistant cell lines derived from a same drug sensitive cell line.

Authors :: Moreira MA
Bagni C
de Pinho MB
Mac-Cormick TM
dos Santos Mota M
Pinto-Silva FE
Daflon-Yunes N
Rumjanek VM
Source :: Leukemia research [Leuk Res] 2014 Aug; Vol. 38 (8), pp. 983-7. Date of Electronic Publication: 2014 Jun 12.
Publication Year :: 2014
Abstract: Resistance to chemotherapy is one of the most relevant aspects of treatment failure in cancer. Cell lines are used as models to study resistance. We analyzed the transcriptional profile of two multidrug resistant (MDR) cell lines (Lucena 1 and FEPS) derived from the same drug-sensitive cell K562. Microarray data identified 130 differentially expressed genes (DEG) between K562 vs. Lucena 1, 1932 between K562 vs. FEPS, and 1211 between Lucena 1 versus FEPS. The NOTCH pathway was affected in FEPS with overexpression of NOTCH2 and HEY1. The highly overexpressed gene in MDR cell lines was ABCB1, and both presented the ABCB1 promoter unmethylated.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Subjects :: ATP Binding Cassette Transporter, Subfamily B
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Cell Line, Tumor
DNA Methylation
Gene Expression Regulation, Leukemic
Humans
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology
Microarray Analysis
Promoter Regions, Genetic
Drug Resistance, Multiple genetics
Drug Resistance, Neoplasm genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics
Transcriptome drug effects

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