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Gatekeeper tyrosine phosphorylation is autoinhibitory for Symbiosis Receptor Kinase.
- Source :
-
FEBS letters [FEBS Lett] 2014 Aug 25; Vol. 588 (17), pp. 2881-9. Date of Electronic Publication: 2014 Jul 01. - Publication Year :
- 2014
-
Abstract
- Plant receptor-like kinases (RLKs) are distinguished by having a tyrosine in the 'gatekeeper' position. Previously we reported Symbiosis Receptor Kinase from Arachis hypogaea (AhSYMRK) to autophosphorylate on the gatekeeper tyrosine (Y670), though this phosphorylation was not necessary for the kinase activity. Here we report that recombinant catalytic domain of AhSYMRK with a phosphomimic substitution in the gatekeeper position (Y670E) is catalytically almost inactive and is conformationally quite distinct from the corresponding native enzyme. Additionally, we show that gatekeeper-phosphorylated AhSYMRK polypeptides are inactive and depletion of this inactive form leads to activation of intramolecular autophosphorylation of AhSYMRK. Together, our results suggest gatekeeper tyrosine autophosphorylation to be autoinhibitory for AhSYMRK.<br /> (Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 588
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 24996184
- Full Text :
- https://doi.org/10.1016/j.febslet.2014.06.056