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Bacterial endophyte Sphingomonas sp. LK11 produces gibberellins and IAA and promotes tomato plant growth.
- Source :
-
Journal of microbiology (Seoul, Korea) [J Microbiol] 2014 Aug; Vol. 52 (8), pp. 689-95. Date of Electronic Publication: 2014 Jul 04. - Publication Year :
- 2014
-
Abstract
- Plant growth promoting endophytic bacteria have been identified as potential growth regulators of crops. Endophytic bacterium, Sphingomonas sp. LK11, was isolated from the leaves of Tephrosia apollinea. The pure culture of Sphingomonas sp. LK11 was subjected to advance chromatographic and spectroscopic techniques to extract and isolate gibberellins (GAs). Deuterated standards of [17, 17-(2)H2]-GA4, [17, 17-(2)H2]-GA9 and [17, 17-(2)H2]-GA20 were used to quantify the bacterial GAs. The analysis of the culture broth of Sphingomonas sp. LK11 revealed the existence of physiologically active gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactive GA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). The endophyte also produced indole acetic acid (11.23 ± 0.93 μM/ml). Tomato plants inoculated with endophytic Sphingomonas sp. LK11 showed significantly increased growth attributes (shoot length, chlorophyll contents, shoot, and root dry weights) compared to the control. This indicated that such phyto-hormones-producing strains could help in increasing crop growth.
- Subjects :
- Chlorophyll biosynthesis
Endophytes isolation & purification
Endophytes metabolism
Gibberellins chemistry
Solanum lycopersicum microbiology
Plant Development
Plant Leaves microbiology
Plant Roots growth & development
Polymerase Chain Reaction
Sphingomonas chemistry
Gibberellins isolation & purification
Gibberellins metabolism
Indoleacetic Acids metabolism
Solanum lycopersicum growth & development
Sphingomonas isolation & purification
Sphingomonas metabolism
Tephrosia microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1976-3794
- Volume :
- 52
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of microbiology (Seoul, Korea)
- Publication Type :
- Academic Journal
- Accession number :
- 24994010
- Full Text :
- https://doi.org/10.1007/s12275-014-4002-7