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Microtubule-associated protein tau. Identification of a novel peptide from bovine brain.
Microtubule-associated protein tau. Identification of a novel peptide from bovine brain.
- Source :
-
FEBS letters [FEBS Lett] 1989 May 08; Vol. 248 (1-2), pp. 87-91. - Publication Year :
- 1989
-
Abstract
- The microtubule-associated protein tau isolated from bovine brain was cleaved with CNBr and the 3 largest peptides of approx. 21, 19 and 18 kDa were obtained. Dephosphorylation of the CNBr digest of tau with alkaline phosphatase changed the electrophoretic mobility of these peptides to 19, 18 and 17 kDa. Amino acid sequencing of the total CNBr digest of tau revealed at least 3 sequences, two of which were highly homologous to previously published mouse and human tau sequences derived from cDNAs. A third amino acid sequence of 17 residues with heterogeneity at position 11 showed no homology with the cDNA-derived tau sequences. These studies suggest that the amino acid sequences of mammalian tau predicted from their cDNAs might be incomplete.
- Subjects :
- Amino Acid Sequence
Amino Acids analysis
Animals
Cattle
Cyanogen Bromide
DNA analysis
Electrophoresis, Polyacrylamide Gel
Humans
Mice
Microtubule-Associated Proteins classification
Microtubule-Associated Proteins genetics
Molecular Sequence Data
Molecular Structure
Phosphorylation
Species Specificity
tau Proteins
Brain Chemistry
Microtubule-Associated Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 248
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 2498127
- Full Text :
- https://doi.org/10.1016/0014-5793(89)80437-5