Start Over

Increased centrosome amplification in aged stem cells of the Drosophila midgut.

Authors :: Park JS
Pyo JH
Na HJ
Jeon HJ
Kim YS
Arking R
Yoo MA
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 Jul 25; Vol. 450 (2), pp. 961-5. Date of Electronic Publication: 2014 Jun 24.
Publication Year :: 2014
Abstract: Age-related changes in long-lived tissue-resident stem cells may be tightly linked to aging and age-related diseases such as cancer. Centrosomes play key roles in cell proliferation, differentiation and migration. Supernumerary centrosomes are known to be an early event in tumorigenesis and senescence. However, the age-related changes of centrosome duplication in tissue-resident stem cells in vivo remain unknown. Here, using anti-γ-tubulin and anti-PH3, we analyzed mitotic intestinal stem cells with supernumerary centrosomes in the adult Drosophila midgut, which may be a versatile model system for stem cell biology. The results showed increased centrosome amplification in intestinal stem cells of aged and oxidatively stressed Drosophila midguts. Increased centrosome amplification was detected by overexpression of PVR, EGFR, and AKT in intestinal stem cells/enteroblasts, known to mimic age-related changes including hyperproliferation of intestinal stem cells and hyperplasia in the midgut. Our data show the first direct evidence for the age-related increase of centrosome amplification in intestinal stem cells and suggest that the Drosophila midgut is an excellent model for studying molecular mechanisms underlying centrosome amplification in aging adult stem cells in vivo.<br /> (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)