Back to Search
Start Over
HopW1 from Pseudomonas syringae disrupts the actin cytoskeleton to promote virulence in Arabidopsis.
- Source :
-
PLoS pathogens [PLoS Pathog] 2014 Jun 26; Vol. 10 (6), pp. e1004232. Date of Electronic Publication: 2014 Jun 26 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- A central mechanism of virulence of extracellular bacterial pathogens is the injection into host cells of effector proteins that modify host cellular functions. HopW1 is an effector injected by the type III secretion system that increases the growth of the plant pathogen Pseudomonas syringae on the Columbia accession of Arabidopsis. When delivered by P. syringae into plant cells, HopW1 causes a reduction in the filamentous actin (F-actin) network and the inhibition of endocytosis, a known actin-dependent process. When directly produced in plants, HopW1 forms complexes with actin, disrupts the actin cytoskeleton and inhibits endocytosis as well as the trafficking of certain proteins to vacuoles. The C-terminal region of HopW1 can reduce the length of actin filaments and therefore solubilize F-actin in vitro. Thus, HopW1 acts by disrupting the actin cytoskeleton and the cell biological processes that depend on actin, which in turn are needed for restricting P. syringae growth in Arabidopsis.
- Subjects :
- Actin Cytoskeleton chemistry
Actin Cytoskeleton drug effects
Actins antagonists & inhibitors
Actins chemistry
Actins genetics
Arabidopsis drug effects
Arabidopsis genetics
Arabidopsis metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Proteins pharmacology
Endocytosis drug effects
Herbicides chemistry
Herbicides metabolism
Herbicides pharmacology
Peptide Fragments chemistry
Peptide Fragments genetics
Peptide Fragments metabolism
Peptide Fragments pharmacology
Plant Immunity drug effects
Plant Proteins antagonists & inhibitors
Plant Proteins chemistry
Plant Proteins metabolism
Plants, Genetically Modified cytology
Plants, Genetically Modified drug effects
Plants, Genetically Modified metabolism
Plants, Genetically Modified microbiology
Protein Interaction Domains and Motifs
Protein Transport drug effects
Pseudomonas syringae immunology
Pseudomonas syringae metabolism
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Recombinant Proteins pharmacology
Seedlings drug effects
Seedlings genetics
Seedlings metabolism
Seedlings microbiology
Solubility
Nicotiana drug effects
Nicotiana genetics
Nicotiana metabolism
Nicotiana microbiology
Virulence drug effects
Virulence Factors chemistry
Virulence Factors genetics
Virulence Factors pharmacology
Actin Cytoskeleton metabolism
Arabidopsis microbiology
Bacterial Proteins metabolism
Host-Pathogen Interactions
Pseudomonas syringae pathogenicity
Virulence Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7374
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PLoS pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 24968323
- Full Text :
- https://doi.org/10.1371/journal.ppat.1004232