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Stable knockdown of protein kinase CK2-alpha (CK2α) inhibits migration and invasion and induces inactivation of hedgehog signaling pathway in hepatocellular carcinoma Hep G2 cells.
- Source :
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Acta histochemica [Acta Histochem] 2014 Oct; Vol. 116 (8), pp. 1501-8. Date of Electronic Publication: 2014 Jun 21. - Publication Year :
- 2014
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Abstract
- Protein kinase CK2-alpha (CK2α), one isoform of the catalytic subunits of serine/threonine kinase CK2, has been indicated to participate in tumorigenesis of various malignancies, including hepatocellular carcinoma (HCC). In the present study, in order to explore the potential role of CK2α in human HCC, we employed short hairpin RNA (shRNA)-mediated RNA interference (RNAi) technology to inhibit the endogenous CK2α expression in HCC cells and established a Hep G2 cell line with stable knockdown of CK2α. Results from wound healing and transwell invasion assays indicated that stable knockdown of CK2α markedly inhibited Hep G2 cell migration and invasion as compared with those transfected with a negative control plasmid. This alteration was accompanied with expression down-regulation of matrix metalloproteinase (MMP)-2, MMP-9, Snail, Slug, Vimentin, and up-regulation of epithelial cadherin (E-cadherin). Moreover, CK2α silencing also induced inactivation of Hedgehog signaling pathway by inhibiting Gli1 and Patched homolog 1 (PTCH1) expressions in HCC cells. Collectively, these results demonstrate that knockdown of CK2α can suppress cell migration and invasion, reduces expression of MMPs, inhibits epithelial-mesenchymal transition (EMT) process and induces inactivation of Hedgehog pathway in HCC cells in vitro. Our study provides in vitro evidence to demonstrate that the pathogenesis of human HCC may be correlated with the high expression of CK2α.<br /> (Copyright © 2014 Elsevier GmbH. All rights reserved.)
- Subjects :
- Casein Kinase II genetics
Cell Movement genetics
Cell Movement physiology
Epithelial-Mesenchymal Transition genetics
Epithelial-Mesenchymal Transition physiology
Hedgehog Proteins genetics
Hep G2 Cells
Humans
Matrix Metalloproteinase 2 genetics
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
Signal Transduction genetics
Signal Transduction physiology
Snail Family Transcription Factors
Transcription Factors genetics
Transcription Factors metabolism
Vimentin genetics
Vimentin metabolism
Casein Kinase II metabolism
Hedgehog Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0372
- Volume :
- 116
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Acta histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 24958341
- Full Text :
- https://doi.org/10.1016/j.acthis.2014.06.001