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Near infrared spectroscopy for rapid determination of Mankin score components: a potential tool for quantitative characterization of articular cartilage at surgery.
- Source :
-
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association [Arthroscopy] 2014 Sep; Vol. 30 (9), pp. 1146-55. Date of Electronic Publication: 2014 Jun 17. - Publication Year :
- 2014
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Abstract
- Purpose: The purpose of this study was to demonstrate the potential of near infrared (NIR) spectroscopy for characterizing the health and degenerative state of articular cartilage based on the components of the Mankin score.<br />Methods: Three models of osteoarthritic degeneration induced in laboratory rats by anterior cruciate ligament (ACL) transection, meniscectomy (MSX), and intra-articular injection of monoiodoacetate (1 mg) (MIA) were used in this study. Degeneration was induced in the right knee joint; each model group consisted of 12 rats (N = 36). After 8 weeks, the animals were euthanized and knee joints were collected. A custom-made diffuse reflectance NIR probe of 5-mm diameter was placed on the tibial and femoral surfaces, and spectral data were acquired from each specimen in the wave number range of 4,000 to 12,500 cm(-1). After spectral data acquisition, the specimens were fixed and safranin O staining (SOS) was performed to assess disease severity based on the Mankin scoring system. Using multivariate statistical analysis, with spectral preprocessing and wavelength selection technique, the spectral data were then correlated to the structural integrity (SI), cellularity (CEL), and matrix staining (SOS) components of the Mankin score for all the samples tested.<br />Results: ACL models showed mild cartilage degeneration, MSX models had moderate degeneration, and MIA models showed severe cartilage degenerative changes both morphologically and histologically. Our results reveal significant linear correlations between the NIR absorption spectra and SI (R(2) = 94.78%), CEL (R(2) = 88.03%), and SOS (R(2) = 96.39%) parameters of all samples in the models. In addition, clustering of the samples according to their level of degeneration, with respect to the Mankin components, was also observed.<br />Conclusions: NIR spectroscopic probing of articular cartilage can potentially provide critical information about the health of articular cartilage matrix in early and advanced stages of osteoarthritis (OA).<br />Clinical Relevance: This rapid nondestructive method can facilitate clinical appraisal of articular cartilage integrity during arthroscopic surgery.<br /> (Copyright © 2014 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Anterior Cruciate Ligament surgery
Anterior Cruciate Ligament Injuries
Cartilage Diseases etiology
Cartilage, Articular drug effects
Femur pathology
Injections, Intra-Articular
Iodoacetic Acid
Knee Joint pathology
Male
Menisci, Tibial surgery
Osteoarthritis etiology
Rats
Tibia pathology
Cartilage Diseases pathology
Cartilage, Articular pathology
Osteoarthritis pathology
Spectroscopy, Near-Infrared
Tibial Meniscus Injuries
Subjects
Details
- Language :
- English
- ISSN :
- 1526-3231
- Volume :
- 30
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Publication Type :
- Academic Journal
- Accession number :
- 24951136
- Full Text :
- https://doi.org/10.1016/j.arthro.2014.04.097