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Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
- Source :
-
PloS one [PLoS One] 2014 Jun 19; Vol. 9 (6), pp. e100430. Date of Electronic Publication: 2014 Jun 19 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
- Subjects :
- Animals
Aspergillosis complications
Aspergillosis microbiology
Aspergillosis pathology
Aspergillus fumigatus cytology
Aspergillus fumigatus growth & development
Chitin metabolism
Colony Count, Microbial
Disease Models, Animal
Humans
Mice, Inbred BALB C
Neutropenia complications
Neutropenia microbiology
Neutropenia pathology
Pneumonia complications
Pneumonia microbiology
Pneumonia pathology
Virulence
beta-Glucans metabolism
Aspergillus fumigatus isolation & purification
Aspergillus fumigatus pathogenicity
Cell Wall metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24945802
- Full Text :
- https://doi.org/10.1371/journal.pone.0100430