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Anticancer effect of total annonaceous acetogenins on hepatocarcinoma.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2015 Sep; Vol. 21 (9), pp. 682-8. Date of Electronic Publication: 2014 Jun 18. - Publication Year :
- 2015
-
Abstract
- Objective: To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma.<br />Methods: The inhibitory effect of TAAs was demonstrated in H22-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis.<br />Results: The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 µg/mL (15.13-26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs.<br />Conclusion: TAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis.
- Subjects :
- Acetogenins chemistry
Acetogenins pharmacology
Animals
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis drug effects
Carcinoma, Hepatocellular enzymology
Carcinoma, Hepatocellular pathology
Caspases metabolism
Cell Cycle drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Humans
Liver Neoplasms enzymology
Liver Neoplasms pathology
Male
Membrane Potential, Mitochondrial drug effects
Mice
Organ Specificity drug effects
Spleen drug effects
Thymus Gland drug effects
Xenograft Model Antitumor Assays
Acetogenins therapeutic use
Annona chemistry
Antineoplastic Agents, Phytogenic therapeutic use
Carcinoma, Hepatocellular drug therapy
Liver Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1672-0415
- Volume :
- 21
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24938450
- Full Text :
- https://doi.org/10.1007/s11655-014-1845-z