Back to Search
Start Over
Therapeutic potential of adipose-derived stromal cells in age-related osteoporosis.
- Source :
-
Biomaterials [Biomaterials] 2014 Aug; Vol. 35 (26), pp. 7326-35. Date of Electronic Publication: 2014 Jun 02. - Publication Year :
- 2014
-
Abstract
- Adipose-derived stromal cells (ASCs) are increasingly being used for orthopedic-based tissue engineering approaches due to their ability to readily undergo osteogenic differentiation. In the present study, we used in vitro and in vivo approaches to evaluate the use of ASCs as a treatment strategy for age-related osteoporosis. Molecular, histological and micro-computed tomography (micro-CT) based approaches confirmed that ASCs isolated from 18-week-old osteoporotic senescence-accelerated mice (SAMP6) were capable of undergoing osteogenesis when cultured in either silk fibroin (SF) scaffolds or scaffold-free microtissues (ASC-MT). A single intratibial injection of CM-Dil-labeled isogeneic ASCs or ASC-MT into SAMP6 recipients significantly improved trabecular bone quality after 6 weeks in comparison to untreated contralateral bones, as determined by micro-CT. Injected ASCs could be observed in paraffin wax bone sections at 24 h and 6 weeks post treatment and induced a significant increase in several molecular markers of bone turnover. Furthermore, a significant improvement in the osteogenic potential of osteoporotic patient-derived human bone marrow stromal cells (BMSCs) was observed when differentiated in conditioned culture media harvested from osteoporotic patient-derived human ASCs. These findings therefore support the use of ASCs as an autologous cell-based approach for the treatment of osteoporosis.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Age Factors
Animals
Cell Differentiation
Cells, Cultured
Humans
Male
Mesenchymal Stem Cells cytology
Mice
Osteoporosis epidemiology
Osteoporosis pathology
Stromal Cells cytology
Tibia cytology
Tibia pathology
Adipose Tissue cytology
Osteogenesis
Osteoporosis therapy
Stromal Cells transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 35
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 24933514
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2014.05.016