Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment.

Authors :: Durant TJ
Dyment N
McCarthy MB
Cote MP
Arciero RA
Mazzocca AD
Rowe D
Source :: Muscles, ligaments and tendons journal [Muscles Ligaments Tendons J] 2014 May 08; Vol. 4 (1), pp. 46-51. Date of Electronic Publication: 2014 May 08 (Print Publication: 2014).
Publication Year :: 2014
Abstract: Introduction: cell-based tissue engineering strategies using human mesenchymal stem cells (hMSCs) may help to augment tendon healing. To further investigate the in-vitro behavior of this cell population, we investigated low oxygen culture levels, and growth factor supplementation and their effect on expression of tendon extracellular proteins and cell proliferation.<br />Materials and Methods: bone marrow aspirate (BMA) was harvested during arthroscopic rotator cuff repair. Characterized hMSCs derived from BMA were incorporated into 3-dimensional tissue engineered constructs (TECs). TECs were analyzed by frozen sections with immunohistochemistry for cell density, collagen I and collagen III expression.<br />Results: growth factor stimulation and low oxygen increased cell density within TECs. Low oxygen and addition of growth factors to culture media demonstrated an increase in collagen I and III expression, both in ambient oxygen conditions and low oxygen conditions.<br />Conclusion: low oxygen and TGFβ3 demonstrated a positive effect on cell number, and type I and III collagen expression in 3D culture environments.

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