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Molecular diversity of the methanotrophic bacteria communities associated with disused tin-mining ponds in Kampar, Perak, Malaysia.
- Source :
-
World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2014 Oct; Vol. 30 (10), pp. 2645-53. Date of Electronic Publication: 2014 Jun 15. - Publication Year :
- 2014
-
Abstract
- In a previous study, notable differences of several physicochemical properties, as well as the community structure of ammonia oxidizing bacteria as judged by 16S rRNA gene analysis, were observed among several disused tin-mining ponds located in the town of Kampar, Malaysia. These variations were associated with the presence of aquatic vegetation as well as past secondary activities that occurred at the ponds. Here, methane oxidizing bacteria (MOB), which are direct participants in the nutrient cycles of aquatic environments and biological indicators of environmental variations, have been characterised via analysis of pmoA functional genes in the same environments. The MOB communities associated with disused tin-mining ponds that were exposed to varying secondary activities were examined in comparison to those in ponds that were left to nature. Comparing the sequence and phylogenetic analysis of the pmoA clone libraries at the different ponds (idle, lotus-cultivated and post-aquaculture), we found pmoA genes indicating the presence of type I and type II MOB at all study sites, but type Ib sequences affiliated with the Methylococcus/Methylocaldum lineage were most ubiquitous (46.7 % of clones). Based on rarefaction analysis and diversity indices, the disused mining pond with lotus culture was observed to harbor the highest richness of MOB. However, varying secondary activity or sample type did not show a strong variation in community patterns as compared to the ammonia oxidizers in our previous study.
- Subjects :
- Bacterial Proteins genetics
Evolution, Molecular
Geologic Sediments microbiology
Malaysia
Methylococcaceae genetics
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Methane metabolism
Methylococcaceae classification
Methylococcaceae isolation & purification
Mining
Ponds microbiology
Tin
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0972
- Volume :
- 30
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- World journal of microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 24929362
- Full Text :
- https://doi.org/10.1007/s11274-014-1687-z