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Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain.
- Source :
-
Cerebral cortex (New York, N.Y. : 1991) [Cereb Cortex] 2015 Oct; Vol. 25 (10), pp. 3290-302. Date of Electronic Publication: 2014 Jun 13. - Publication Year :
- 2015
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Abstract
- The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is much debated. Intermediate progenitors in the cortical subventricular zone are defined by expression of T-box brain-2 (Tbr2). In this study we demonstrate by using the Tbr2(Cre) mouse line and state-of-the-art cell lineage labeling techniques, that IPC derived cells contribute substantial proportions 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. We also describe the laminar dispersion of clonally derived cells from IPCs using a recently described clonal analysis tool (CLoNe) and show that pair-generated cells in different layers cluster closer (142.1 ± 76.8 μm) than unrelated cells (294.9 ± 105.4 μm). The clonal dispersion from individual Tbr2 positive intermediate progenitors contributes to increasing the cortical surface. Our study also describes extracortical contributions from Tbr2+ progenitors to the lateral olfactory tract and ventromedial hypothalamic nucleus.<br /> (© The Author 2014. Published by Oxford University Press.)
- Subjects :
- Animals
Astrocytes metabolism
Astrocytes physiology
Brain embryology
Brain metabolism
Cerebral Cortex metabolism
GABAergic Neurons metabolism
GABAergic Neurons physiology
Lateral Ventricles metabolism
Lateral Ventricles physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neural Stem Cells metabolism
Stem Cells metabolism
T-Box Domain Proteins genetics
Cerebral Cortex embryology
Neural Stem Cells physiology
Stem Cells physiology
T-Box Domain Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2199
- Volume :
- 25
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cerebral cortex (New York, N.Y. : 1991)
- Publication Type :
- Academic Journal
- Accession number :
- 24927931
- Full Text :
- https://doi.org/10.1093/cercor/bhu125