Back to Search
Start Over
Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat-inactivated Mycobacterium bovis.
- Source :
-
Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2014 Aug; Vol. 21 (8), pp. 1128-36. Date of Electronic Publication: 2014 Jun 11. - Publication Year :
- 2014
-
Abstract
- Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-β), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.<br /> (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Administration, Oral
Animals
Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Complement C3 genetics
Enzyme-Linked Immunosorbent Assay
Interferon-beta genetics
Leukocytes, Mononuclear metabolism
Methylmalonyl-CoA Mutase genetics
Mouth Mucosa immunology
RNA, Messenger biosynthesis
Receptors, CCR7 genetics
Sus scrofa
Tuberculosis, Pulmonary diagnosis
Tuberculosis, Pulmonary microbiology
Vaccination
Vaccines, Inactivated administration & dosage
Vaccines, Inactivated immunology
Mycobacterium bovis immunology
Palatine Tonsil immunology
Tuberculosis Vaccines immunology
Tuberculosis, Pulmonary immunology
Tuberculosis, Pulmonary veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1556-679X
- Volume :
- 21
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical and vaccine immunology : CVI
- Publication Type :
- Academic Journal
- Accession number :
- 24920604
- Full Text :
- https://doi.org/10.1128/CVI.00221-14