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Physicochemical characterization of solid lipid nanoparticles (SLNs) prepared by a novel microemulsion technique.
- Source :
-
Journal of colloid and interface science [J Colloid Interface Sci] 2014 Aug 15; Vol. 428, pp. 286-94. Date of Electronic Publication: 2014 May 04. - Publication Year :
- 2014
-
Abstract
- Hypothesis: Solid lipid nanoparticles (SLNs) produced by conventional microemulsion techniques using thermal heat have specific limitations (e.g. high polydispersity, instability and low encapsulation). Replacing thermal heat with microwave heat may produce SLNs which overcome some of these limitations.<br />Experiments: Stearic acid-based SLNs prepared with Tween® 20 as the emulsifier were chosen as the optimum formulation to encapsulate and potentially deliver the antibacterial drug tetracycline. All formulations were characterized for their particle size, zeta potential, encapsulation efficiency, loading capacity, thermal and X-ray diffraction analyses. Short-term stability and in vitro drug studies were also performed.<br />Findings: Microwave heating helps to overcome several disadvantages associated with thermal heating (nonuniform, inefficient and slow) and results in improved particle characteristics. There is thus the potential for new opportunities in the development of colloidal carriers. The particle sizes of microwave-produced SLNs were in the desired nanometer range (200-250 nm) with both lower size and lower polydispersity than the conventional SLNs. We take this as an indication of improved stability; however zeta potential measurements were not different, indicating similar stability. True stability testing (visual observation with time) did show that the microwave-induced SLNs were found to be more stable, particularly when refrigerated. The microwave-produced SLNs also demonstrated improved encapsulation efficiency and loading capacity. Thermal and diffraction analysis confirmed a lowered crystallinity of stearic acid with successful incorporation of tetracycline into the SLNs. In vitro release studies indicated that, after an initial burst release, SLNs could provide prolonged release of tetracycline. The presence of tetracycline and non-toxicity of carriers towards microbes was confirmed by antimicrobial susceptibility tests.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents pharmacology
Escherichia coli drug effects
Escherichia coli Infections drug therapy
Humans
Microwaves
Polysorbates chemistry
Staphylococcal Infections drug therapy
Staphylococcus aureus drug effects
Tetracycline pharmacology
Anti-Bacterial Agents administration & dosage
Drug Carriers chemistry
Emulsions chemistry
Nanoparticles chemistry
Stearic Acids chemistry
Tetracycline administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1095-7103
- Volume :
- 428
- Database :
- MEDLINE
- Journal :
- Journal of colloid and interface science
- Publication Type :
- Academic Journal
- Accession number :
- 24910064
- Full Text :
- https://doi.org/10.1016/j.jcis.2014.04.057