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Decreased cellular levels of palmitic amide are linked to 5-fluorouracil resistance in human colon cancer cells.
- Source :
-
Hepato-gastroenterology [Hepatogastroenterology] 2014 Mar-Apr; Vol. 61 (130), pp. 343-8. - Publication Year :
- 2014
-
Abstract
- Background/aims: The aim of this study was to investigate whether profiling metabolic compounds in human colon cancer cells with induced 5-florouracil resistance enables identification of predictive biomarkers for 5-florouracil resistance.<br />Methodology: 5-florouracil resistant and parental cells were extracted using methanol/chloroform solution, and analyzed by MALDI-TOF. Principal components analysis and discriminant analysis was performed to select low-mass ions with strong discriminating power between 5-florouracil resistant and parental cells. The correlation between the intensities of low-mass ions and intrinsic 5-florouracil resistance in 11 colon cancer cells was analyzed using the Spearman rank coefficient.<br />Results: Eleven low-mass ions had strong discrimi-nating power between 5-florouracil-resistant and parental cells. Of these, the intensity of a low-mass ion with 256.29 m/z was negatively correlated with intrinsic 5-florouracil resistance in 11 colon cancer cells (r = -0.6545, P = 0.0338). By searching the H+ adduct with 0.05 m/z tolerance in the Human Metabolome Database, a low-mass ion of 256.29 m/z was identified as palmitic amide. Interestingly, extracellular treatment with palmitic amide reduced 5-florouracil resistance and invasiveness in 5-florouracil-resistant cells.<br />Conclusions: Palmitic amide showed potential not only as a predictor of 5-florouracil resistance, but also for reduction of 5-florouracil resistance in colon cancer cells.
- Subjects :
- Biomarkers, Tumor metabolism
Cell Line, Tumor
Drug Resistance, Neoplasm
Drug Synergism
Fluorouracil administration & dosage
Humans
Palmitic Acids administration & dosage
Principal Component Analysis
Signal Transduction drug effects
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Statistics, Nonparametric
Antineoplastic Combined Chemotherapy Protocols pharmacology
Colonic Neoplasms drug therapy
Colonic Neoplasms metabolism
Fluorouracil pharmacology
Palmitic Acids metabolism
Palmitic Acids pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0172-6390
- Volume :
- 61
- Issue :
- 130
- Database :
- MEDLINE
- Journal :
- Hepato-gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 24901137