Back to Search
Start Over
Characterization of SET/I2PP2A isoforms in dogs.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2014 Sep; Vol. 76 (9), pp. 1235-40. Date of Electronic Publication: 2014 Jun 03. - Publication Year :
- 2014
-
Abstract
- SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Line, Tumor
Cloning, Molecular
Dog Diseases enzymology
Dog Diseases genetics
Dogs
Lymphoma enzymology
Lymphoma genetics
Microscopy, Confocal veterinary
Molecular Sequence Data
Oncogene Proteins genetics
Protein Isoforms metabolism
Protein Phosphatase 2 antagonists & inhibitors
Sequence Alignment
Sequence Analysis, DNA
Transfection veterinary
Dog Diseases metabolism
Lymphoma metabolism
Lymphoma veterinary
Oncogene Proteins metabolism
Protein Phosphatase 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 76
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 24897959
- Full Text :
- https://doi.org/10.1292/jvms.14-0209