Back to Search
Start Over
Role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme during ureteric budding.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2014 Aug; Vol. 34 (16), pp. 3096-105. Date of Electronic Publication: 2014 Jun 02. - Publication Year :
- 2014
-
Abstract
- Development of the metanephric kidney begins with the induction of a single ureteric bud (UB) on the caudal Wolffian duct (WD) in response to GDNF (glial cell line-derived neurotrophic factor) produced by the adjacent metanephric mesenchyme (MM). Mutual interaction between the UB and MM maintains expression of GDNF in the MM, thereby supporting further outgrowth and branching morphogenesis of the UB, while the MM also grows and aggregates around the branched tips of the UB. Ror2, a member of the Ror family of receptor tyrosine kinases, has been shown to act as a receptor for Wnt5a to mediate noncanonical Wnt signaling. We show that Ror2 is predominantly expressed in the MM during UB induction and that Ror2- and Wnt5a-deficient mice exhibit duplicated ureters and kidneys due to ectopic UB induction. During initial UB formation, these mutant embryos show dysregulated positioning of the MM, resulting in spatiotemporally aberrant interaction between the MM and WD, which provides the WD with inappropriate GDNF signaling. Furthermore, the numbers of proliferating cells in the mutant MM are markedly reduced compared to the wild-type MM. These results indicate an important role of Wnt5a-Ror2 signaling in morphogenesis of the MM to ensure proper epithelial tubular formation of the UB required for kidney development.<br /> (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Animals
Cell Proliferation
Gene Expression Regulation, Developmental
Mice
Mice, Inbred ICR
Mice, Knockout
Morphogenesis
Organ Culture Techniques
Organogenesis
Receptor Tyrosine Kinase-like Orphan Receptors genetics
Wnt Proteins genetics
Wnt Signaling Pathway
Wnt-5a Protein
Wolffian Ducts embryology
Glial Cell Line-Derived Neurotrophic Factor metabolism
Kidney embryology
Receptor Tyrosine Kinase-like Orphan Receptors metabolism
Ureter embryology
Wnt Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5549
- Volume :
- 34
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 24891614
- Full Text :
- https://doi.org/10.1128/MCB.00491-14