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The angiogenic biomarker endocan is upregulated in proliferative diabetic retinopathy and correlates with vascular endothelial growth factor.
- Source :
-
Current eye research [Curr Eye Res] 2015 Mar; Vol. 40 (3), pp. 321-31. Date of Electronic Publication: 2014 May 28. - Publication Year :
- 2015
-
Abstract
- Purpose/aim: Endocan is a proteoglycan specifically secreted by endothelial cells, is a marker of angiogenesis and endothelial cell activation in response to proangiogenic signals. The aim of this study was to measure the levels of endocan in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate its levels with clinical disease activity and the levels of the angiogenic biomarkers vascular endothelial growth factor (VEGF), soluble vascular endothelial-cadherin (sVE-cadherin) and soluble endoglin (sEng). In addition, we investigated the expression of endocan and correlated it with the level of vascularization in PDR epiretinal membranes.<br />Materials and Methods: Vitreous samples from 44 PDR and 29 non-diabetic patients were studied by enzyme-linked immunosorbent assay. Epiretinal membranes from 14 patients with PDR were studied by immunohistochemistry.<br />Results: Endocan, VEGF, sVE-cadherin and sEng levels were significantly higher in PDR patients than in non-diabetic patients (p < 0.001; p = 0.002; p < 0.001; p = 0.001, respectively). Endocan levels were significantly higher in patients with active PDR than in patients with inactive PDR and non-diabetic patients (p < 0.001). There were significant positive correlations between endocan levels and the levels of VEGF (r = 0.574, p < 0.001) and sVE-cadherin (r = 0.498, p < 0.001). In epiretinal membranes, vascular endothelial cells and myofibroblasts expressed endocan. There was a significant positive correlation between the number of blood vessels expressing CD34 and the number of blood vessels expressing endocan (r = 0.933, p < 0.001).<br />Conclusions: Our findings suggest that upregulation of endocan expression in PDR could be a reflection of endothelial cell activation associated with angiogenesis.
- Subjects :
- Aged
Antigens, CD metabolism
Cadherins metabolism
Diabetic Retinopathy pathology
Diabetic Retinopathy surgery
Endoglin
Enzyme-Linked Immunosorbent Assay
Epiretinal Membrane metabolism
Epiretinal Membrane surgery
Female
Humans
Immunohistochemistry
Male
Middle Aged
Receptors, Cell Surface metabolism
Retinal Neovascularization pathology
Retinal Neovascularization surgery
Up-Regulation
Vitrectomy
Angiogenic Proteins metabolism
Biomarkers metabolism
Diabetic Retinopathy metabolism
Neoplasm Proteins metabolism
Proteoglycans metabolism
Retinal Neovascularization metabolism
Vascular Endothelial Growth Factor A metabolism
Vitreous Body metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2202
- Volume :
- 40
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 24871583
- Full Text :
- https://doi.org/10.3109/02713683.2014.921312