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Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells.
- Source :
-
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology [Neurol Sci] 2014 Nov; Vol. 35 (11), pp. 1707-14. Date of Electronic Publication: 2014 May 29. - Publication Year :
- 2014
-
Abstract
- GPR137 are ubiquitously expressed in the central nervous system. However, the role o f GPR137 in human malignant glioma is still poorly known. In the present study, we firstly detected the expression of GPR137 in 29 human glioma tissue specimens by immunohistochemistry and in 5 malignant glioma cell lines by quantitative RT-PCR. The expression of GPR137 was much stronger in high-grade gliomas than in low-grade gliomas. Lentivirus-mediated small interfering RNAs (siRNAs) were employed to knock down GPR137 expression in glioma cells. Inhibition of GPR137 expression by RNAi significantly inhibited the proliferation and colony-forming capacity of U251, A172 and U373 cells. Moreover, flow cytometry analysis showed that knockdown of GPR137 led to the cell-cycle arrest at the S phase. Our results indicated that GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells.
- Subjects :
- Blotting, Western
Cell Cycle Checkpoints physiology
Cell Line, Tumor
Cell Proliferation physiology
Flow Cytometry
Gene Knockdown Techniques
Humans
Immunohistochemistry
Reverse Transcriptase Polymerase Chain Reaction
Brain Neoplasms pathology
Glioma pathology
Receptors, G-Protein-Coupled metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1590-3478
- Volume :
- 35
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
- Publication Type :
- Academic Journal
- Accession number :
- 24870220
- Full Text :
- https://doi.org/10.1007/s10072-014-1817-2