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OsRACK1 is involved in abscisic acid- and H2O2-mediated signaling to regulate seed germination in rice (Oryza sativa, L.).
- Source :
-
PloS one [PLoS One] 2014 May 27; Vol. 9 (5), pp. e97120. Date of Electronic Publication: 2014 May 27 (Print Publication: 2014). - Publication Year :
- 2014
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Abstract
- The receptor for activated C kinase 1 (RACK1) is one member of the most important WD repeat-containing family of proteins found in all eukaryotes and is involved in multiple signaling pathways. However, compared with the progress in the area of mammalian RACK1, our understanding of the functions and molecular mechanisms of RACK1 in the regulation of plant growth and development is still in its infancy. In the present study, we investigated the roles of rice RACK1A gene (OsRACK1A) in controlling seed germination and its molecular mechanisms by generating a series of transgenic rice lines, of which OsRACK1A was either over-expressed or under-expressed. Our results showed that OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2. Inhibition of ABA biosynthesis had no enhancing effect on germination, whereas inhibition of ABA catabolism significantly suppressed germination. ABA inhibition on seed germination was almost fully recovered by exogenous H2O2 treatment. Quantitative analyses showed that endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1A-down regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines. Quantitative real-time PCR analyses showed that the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines. It is concluded that OsRACK1A positively regulates seed germination by controlling endogenous levels of ABA and H2O2 and their interaction.
- Subjects :
- Blotting, Western
Gene Expression Regulation, Plant
Oryza drug effects
Oryza enzymology
Oxidants pharmacology
Plant Growth Regulators pharmacology
Plants, Genetically Modified drug effects
Plants, Genetically Modified enzymology
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Receptors for Activated C Kinase
Receptors, Cell Surface genetics
Reverse Transcriptase Polymerase Chain Reaction
Seeds chemistry
Abscisic Acid pharmacology
Germination drug effects
Hydrogen Peroxide pharmacology
Oryza growth & development
Plants, Genetically Modified growth & development
Receptors, Cell Surface metabolism
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24865690
- Full Text :
- https://doi.org/10.1371/journal.pone.0097120