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Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays.
- Source :
-
Stem cells translational medicine [Stem Cells Transl Med] 2014 Jul; Vol. 3 (7), pp. 867-78. Date of Electronic Publication: 2014 May 22. - Publication Year :
- 2014
-
Abstract
- The ability to differentiate induced pluripotent stem cells (iPSCs) into committed skeletal progenitors could allow for an unlimited autologous supply of such cells for therapeutic uses; therefore, we attempted to create novel bone-forming cells from human iPSCs using lines from two distinct tissue sources and methods of differentiation that we previously devised for osteogenic differentiation of human embryonic stem cells, and as suggested by other publications. The resulting cells were assayed using in vitro methods, and the results were compared with those obtained from in vivo transplantation assays. Our results show that true bone was formed in vivo by derivatives of several iPSC lines, but that the successful cell lines and differentiation methodologies were not predicted by the results of the in vitro assays. In addition, bone was formed equally well from iPSCs originating from skin or bone marrow stromal cells (also known as bone marrow-derived mesenchymal stem cells), suggesting that the iPSCs did not retain a "memory" of their previous life. Furthermore, one of the iPSC-derived cell lines formed verifiable cartilage in vivo, which likewise was not predicted by in vitro assays.<br /> (©AlphaMed Press.)
- Subjects :
- Aged
Aged, 80 and over
Animals
Cell Line
Cellular Reprogramming
Chondrocytes transplantation
Female
Gene Expression Regulation, Developmental
Humans
Induced Pluripotent Stem Cells transplantation
Male
Mesenchymal Stem Cell Transplantation
Mice
Osteoblasts transplantation
Phenotype
Transfection
Biological Assay methods
Cell Differentiation
Chondrocytes metabolism
Chondrogenesis
Induced Pluripotent Stem Cells metabolism
Mesenchymal Stem Cells metabolism
Osteoblasts metabolism
Osteogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 2157-6564
- Volume :
- 3
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Stem cells translational medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24855277
- Full Text :
- https://doi.org/10.5966/sctm.2013-0154