Back to Search
Start Over
Rapamycin attenuates pulmonary allergic vasculitis in murine model by reducing TGF-β production in the lung.
- Source :
-
Allergology international : official journal of the Japanese Society of Allergology [Allergol Int] 2014 Sep; Vol. 63 (3), pp. 457-66. Date of Electronic Publication: 2014 May 25. - Publication Year :
- 2014
-
Abstract
- Background: Rapamycin has been reported to inhibit mesenchymal cell proliferation in a murine model of pulmonary fibrosis. In the present study, we examined the effects of rapamycin on vascular remodeling including intraluminal myofibroblast proliferation in a murine model of allergic vasculitis with eosinophil infiltration.<br />Methods: C57BL/6 mice were sensitized with ovalbumin (OVA) and alum. The positive controls were exposed to aerosolized OVA daily for 7 days. The other group of mice was administered with rapamycin (1mg/kg) intraperitoneally, in parallel with daily exposure to aerosolized OVA for 7 days. On the 3rd and 7th day, bronchoalveolar lavage (BAL) was performed and the lungs were excised for pathological analysis. Cell differentials were determined and concentrations of IL-4, IL-5, IL-13 and TGF-β in the BAL fluid (BALF) were measured. Semi-quantitative analysis of pathological changes in the pulmonary arteries was evaluated according to the severity of vasculitis.<br />Results: The number of eosinophils in BALF was reduced significantly in the mice treated with rapamycin compared to the positive control. There was a significant decrease in the TGF-β concentration of the BALF in the rapamycin-treated group compared to that of the positive control. The pathological scores were reduced significantly in the rapamycin-treated group compared to the positive control group. Intraluminal myofibroblasts in pulmonary arteries were reduced dramatically in the rapamycin-treated group compared to the positive control group.<br />Conclusions: Rapamycin suppressed pulmonary vascular remodeling in a murine model of allergic vasculitis with eosinophil infiltration through reducing eosinophil infiltration and TGF-β production in the lung and inhibition against biological action of TGF-β.
- Subjects :
- Animals
Cell Proliferation drug effects
Disease Models, Animal
Disease Progression
Eosinophils drug effects
Eosinophils pathology
Female
Humans
Hypersensitivity immunology
Lung Diseases immunology
Mice
Mice, Inbred C57BL
Myofibroblasts drug effects
Myofibroblasts physiology
Pulmonary Artery pathology
Vasculitis immunology
Hypersensitivity drug therapy
Immunosuppressive Agents administration & dosage
Lung Diseases drug therapy
Pulmonary Artery drug effects
Sirolimus administration & dosage
Transforming Growth Factor beta metabolism
Vascular Remodeling drug effects
Vasculitis drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1440-1592
- Volume :
- 63
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Allergology international : official journal of the Japanese Society of Allergology
- Publication Type :
- Academic Journal
- Accession number :
- 24851949
- Full Text :
- https://doi.org/10.2332/allergolint.13-OA-0679