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Differential migration and activation profile of monocytes after trophoblast interaction.
Differential migration and activation profile of monocytes after trophoblast interaction.
- Source :
-
PloS one [PLoS One] 2014 May 21; Vol. 9 (5), pp. e97147. Date of Electronic Publication: 2014 May 21 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Macrophages at the maternal-placental interface coordinate opposite demands under the control of trophoblast cells such as the response against pathogens on one hand, and apoptotic cell clearance and wound healing with the production of suppressor cytokines. Here, we investigated whether trophoblast cells induce maternal monocyte activation towards an alternative activated macrophage profile and whether bacterial or viral stimuli modulate their migratory properties. We used an in vitro model of the maternal-placental interface represented by co-cultures of CD14+ cells isolated from fertile women with first trimester trophoblast cell line (Swan-71 cells) in the presence or absence of pathogen associated molecular pattern (PAMP) stimuli lipopolysaccharide (LPS), peptidoglycan (PGN) or poly [I:C]). Maternal CD14+ cells showed increased CD16 and CD39 expression, both markers associated to an alternative activation profile, with no changes in CD80 expression after trophoblast cell interaction. These changes were accompanied by increased IL-10 and decreased IL-12 production by CD14+ cells. After stimulation with LPS, PGN or poly [I:C], monocytes co-cultured with trophoblast cells had lower production of TNF-α and IL-1β compared with non co-cultured monocytes. Interestingly, monocyte migration towards trophoblast cells was prevented in the presence of LPS or PGN but not after 24h of stimulation with poly [I:C]. LPS or PGN also decreased CCR5, CXCL-8 and CCL5 expression. Finally, trophoblast cells co-cultured with monocytes in the presence of pathological stimuli failed to increase chemokine expression, indicating a bidirectional effect. In conclusion, trophoblast might 'instruct' maternal monocytes to express an alternative activation profile and restrain their early recruitment under pathological threats as one of the first strategies to avoid potential tissue damage at the maternal-placental interface.
- Subjects :
- Antigens, CD genetics
Antigens, CD metabolism
Apyrase genetics
Apyrase metabolism
Cell Communication drug effects
Cell Movement drug effects
Chemokine CCL5 genetics
Chemokine CCL5 metabolism
Coculture Techniques
Female
GPI-Linked Proteins genetics
GPI-Linked Proteins metabolism
Gene Expression drug effects
Humans
Interleukin-1beta genetics
Interleukin-1beta metabolism
Interleukin-8 genetics
Interleukin-8 metabolism
Lipopolysaccharide Receptors genetics
Lipopolysaccharide Receptors metabolism
Monocytes cytology
Monocytes metabolism
Receptors, CCR5 genetics
Receptors, CCR5 metabolism
Receptors, IgG genetics
Receptors, IgG metabolism
Trophoblasts cytology
Trophoblasts metabolism
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Lipopolysaccharides pharmacology
Monocytes drug effects
Peptidoglycan pharmacology
Poly I-C pharmacology
Trophoblasts drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24849800
- Full Text :
- https://doi.org/10.1371/journal.pone.0097147