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Identification of stringent response-related and potential serological proteins released from Bacillus anthracis overexpressing the RelA/SpoT homolog, Rsh Bant.
- Source :
-
Current microbiology [Curr Microbiol] 2014 Oct; Vol. 69 (4), pp. 436-44. Date of Electronic Publication: 2014 May 17. - Publication Year :
- 2014
-
Abstract
- RelA and SpoT synthesize ppGpp, a key effector molecule that facilitates the adaptation of bacteria to nutrient starvation and other stresses, known as the stringent response. To investigate the role of Rsh Bant , a putative RelA/SpoT homolog (encoded by BAS4302) in Bacillus anthracis, we examined the alteration of the secretome profiles after the overexpression of a functional His-Rsh Bant protein in the B. anthracis strain Sterne at the stationary growth phase. In the ppGpp-deficient E. coli mutant strain CF1693, overexpression of Rsh Bant restored a ppGpp-dependent growth defect on minimal glucose media. The secretome profiles obtained using a two-dimensional electrophoresis (2-DE) analysis were altered by overexpression of Rsh Bant in B. anthracis. Among the 66 protein spots differentially expressed >1.5-fold, the 29 proteins were abundant for further identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Functional categorization of those proteins implicated their involvement in various biological activities. Taken together, our results imply that overexpression of a functional His-Rsh Bant can lead to the increased levels of intracellular ppGpp in B. anthracis, resulting in the significant changes in its secretome profiling. The stringent response-controlled proteins identified are likely useful as potential targets for serodiagnostic applications.
- Subjects :
- Bacillus anthracis genetics
Bacillus anthracis metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Secretion Systems
Electrophoresis, Gel, Two-Dimensional
GTP Pyrophosphokinase chemistry
GTP Pyrophosphokinase genetics
Gene Expression Regulation, Bacterial
Proteomics
Bacillus anthracis enzymology
Bacterial Proteins metabolism
GTP Pyrophosphokinase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 69
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24838666
- Full Text :
- https://doi.org/10.1007/s00284-014-0606-8