Back to Search
Start Over
[Preliminary evaluation on the diagnostic performance of five native antigens from Echinococcus granulosus].
- Source :
-
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi] 2013 Oct; Vol. 31 (5), pp. 357-62. - Publication Year :
- 2013
-
Abstract
- Objective: To evaluate the diagnostic potential of 5 native antigens obtained from Echinococcus granulosus.<br />Methods: The cyst fluid and protoscoleces were collected from infected livers of slaughtered sheep near Urumqi. The protoscolex somatic soluble antigen (EgPS), crude hydatid cyst fluid antigen (EgHF), partially purified hydatid fluid antigen (Burstein's antigen, EgBu) and the native antigen B (EgAgB) were prepared. Echinococcus granulosus adult worms were collected from experimentally infected dogs, and then used to prepare adult somatic soluble antigen (EgAS). Serum samples from patients with cystic echinococcosis (369 cases), alveolar echinococcosis (14 cases), cysticercosis (20 cases), schistosomiasis (50 cases), paragonimiasis westermani (10 cases), visceral leishmaniasis (5 cases), and healthy individuals (366 cases) were examined for the antibodies against 5 native antigens with indirect ELISA. A ROC analysis was performed to determine a cut-off value for each native antigen.<br />Results: EgPS presented the highest AUC(BOC) (0.958 +/- 0.067), sensitivity (88.7%), positive predictive value (0.96), positive likelihood ratio (16.61), diagnostic efficiency (91.7%), and odds ratio (138.475). The specificity of EgBu (96.1%) was the highest, followed by EgAgB (95.7%), EgPS (94.7%), EgAS (89.0%), and EgHF (80.4%). The Youden index was ranked in order of EgPS>EgBu>EgHF>EgAS>EgAgB. The antibody reactive intensity of EgBu-ELISA was highest (4.055 +/- 1.365) and the EgAS-ELISA was lowest (2.947 +/- 1.276), and there was no significant difference between them (P > 0.05). The five native antigens presented high cross-reactivity with sera from alveolar echinococcosis (9/14-12/14) and from cysticercosis (8/20-16/20).<br />Conclusion: There are significant differences among the five native antigen-ELISA. The EgPS antigen has the best diagnostic performance , which is followed by EgBu and EgAgB.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Animals
Child
Child, Preschool
Cross Reactions
Dogs
Echinococcosis blood
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Middle Aged
Sensitivity and Specificity
Sheep
Young Adult
Antigens, Helminth classification
Echinococcosis diagnosis
Echinococcus granulosus immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-7423
- Volume :
- 31
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
- Publication Type :
- Academic Journal
- Accession number :
- 24818390