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Cytosolic double-stranded DNA induces nonnecroptotic programmed cell death in trophoblasts via IFI16.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2014 Nov 01; Vol. 210 (9), pp. 1476-86. Date of Electronic Publication: 2014 May 08. - Publication Year :
- 2014
-
Abstract
- The mechanisms underlying the immune defense by trophoblasts against pathogens remain ill defined. We demonstrated that placental cell death was increased upon in vivo exposure to Listeria monocytogenes. The death of infected cells is an important host innate defense mechanism. Meanwhile, double-stranded DNA (dsDNA) derived from intracellular bacteria or dsDNA viruses is emerging as a potent pathogen-associated molecular pattern recognized by host cells. We sought to characterize trophoblast death in response to cytosolic dsDNA challenge. Our results showed that dsDNA induced caspase-dependent and -independent cell death in human trophoblasts. However, necroptosis, a cell death pathway independent of caspase, could not be induced by dsDNA treatment, even in the presence of exogenously expressed RIPK3. L. monocytogenes-derived genomic DNA triggered a similar cell death pattern. Moreover, the cell death in response to dsDNA was IFI16 dependent. These data suggest that cytosolic dsDNA induces nonnecroptotic cell death in trophoblasts via IFI16, and this could contribute to placental barrier against infection.<br /> (© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Caspases metabolism
Cell Line
DNA, Bacterial pharmacology
Female
Humans
Listeria monocytogenes physiology
Listeriosis microbiology
Male
Mice, Inbred C57BL
Pregnancy
Trophoblasts drug effects
Cell Death drug effects
DNA pharmacology
Listeria monocytogenes drug effects
Nuclear Proteins physiology
Phosphoproteins physiology
Trophoblasts microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 210
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 24812048
- Full Text :
- https://doi.org/10.1093/infdis/jiu272