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Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2014 Aug; Vol. 35 (8), pp. 7743-54. Date of Electronic Publication: 2014 May 09. - Publication Year :
- 2014
-
Abstract
- LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.
- Subjects :
- Aged
Animals
Carcinoma, Squamous Cell mortality
Cell Line, Tumor
Cell Movement
Cell Proliferation
Esophageal Neoplasms mortality
Esophageal Squamous Cell Carcinoma
Female
Humans
Male
Mice
Mice, Inbred BALB C
Middle Aged
Neoplasm Invasiveness
Prognosis
RNA, Long Noncoding genetics
Up-Regulation
Carcinoma, Squamous Cell genetics
Esophageal Neoplasms genetics
Intracellular Signaling Peptides and Proteins genetics
Nerve Tissue Proteins genetics
RNA, Long Noncoding physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 35
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24810925
- Full Text :
- https://doi.org/10.1007/s13277-014-2013-y