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Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines.
- Source :
-
Virus genes [Virus Genes] 2014 Aug; Vol. 49 (1), pp. 30-7. Date of Electronic Publication: 2014 May 03. - Publication Year :
- 2014
-
Abstract
- The hepatitis B virus (HBV) genomes in Taiwanese aborigines, whose ancestors have lived in Taiwan for over 10,000 years, have not been characterized. In order to characterize of HBV in this special population, serum samples were obtained from serologically HBsAg-positive 27 Taiwanese aborigines. The pre-S1/S2 region and the full-length 3.2 kb of the HBV genome were amplified by PCR. Obtained amplicons were sequenced and confirmed the HBV genotypes by phylogenetic analysis. By phylogenetic analysis of the sequence of pre-S1/pre-S2 region, HBV/B2 (21/27: 78 %) was the most prevalent followed by genotype D (6/27: 22 %). Two strains of HBV/B2, each having 3,215 bp genomes, had recombination with genotype C in the pre-C/C gene which is characteristic of subgenotype B2 circulating in Southeast Asia. Interestingly, six strains of genotype D formed a distinct cluster between subgenotypes D1 and D2 suggesting a novel group of HBV. A similar finding could also be confirmed based on the entire 3,182 bp genome from four strains of HBV/D. This new cluster was supported by a branch with 99 % bootstrap value and 3.4-5.8 % nucleotide divergence over the entire genome from other known subgenotypes D1 to D9. Four strains of the new D subgenotype showed serotype ayw2, but had unique amino acid sequences consisting of N115 in the preS/S gene; P41 in the X gene; S239, K/E295, V567, and P708 in the P gene, respectively. From the above results, we provisionally proposed to designate it as novel quasi-subgenotype D2 identified in Taiwanese aborigines.
- Subjects :
- Adult
Aged
Aged, 80 and over
Cluster Analysis
DNA, Viral chemistry
DNA, Viral genetics
Female
Genome, Viral
Genotype
Hepatitis B Surface Antigens genetics
Hepatitis B virus isolation & purification
History, Medieval
Humans
Male
Middle Aged
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Population Groups
Sequence Analysis, DNA
Sequence Homology
Taiwan
Hepatitis B virology
Hepatitis B virus classification
Hepatitis B virus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1572-994X
- Volume :
- 49
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virus genes
- Publication Type :
- Academic Journal
- Accession number :
- 24792512
- Full Text :
- https://doi.org/10.1007/s11262-014-1072-x