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SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis.
- Source :
-
Journal of experimental botany [J Exp Bot] 2014 Jul; Vol. 65 (12), pp. 3299-310. Date of Electronic Publication: 2014 Apr 30. - Publication Year :
- 2014
-
Abstract
- Proteins containing the SPX domain are believed to play vital roles in the phosphorus (P) signalling network in plants. However, the functions of SPX proteins in legumes remain largely unknown. In this study, three SPX members, PvSPX1-PvSPX3 were cloned from common bean (Phaseolus vulgaris L.). It was found that the transcripts of all three PvSPX members were significantly enhanced in both bean leaves and roots by phosphate (Pi) starvation. Among them, the expression of nuclear localized PvSPX1 showed more sensitive and rapid responses to Pi starvation. Consistently, only overexpression of PvSPX1 resulted in increased root P concentration and modified morphology of transgenic bean hairy roots, such as inhibited root growth and an enlarged root hair zone. It was further demonstrated that PvSPX1 transcripts were up-regulated by overexpressing PvPHR1, and overexpressing PvSPX1 led to increased transcripts of 10 Pi starvation-responsive genes in transgenic bean hairy roots. Taken together, it is suggested that PvSPX1 is a positive regulator in the P signalling network of common bean, and is downstream of PvPHR1.<br /> (© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Subjects :
- Homeostasis
Molecular Sequence Data
Phaseolus cytology
Phaseolus genetics
Phosphates deficiency
Phylogeny
Plant Proteins genetics
Plant Roots cytology
Plant Roots genetics
Plant Roots physiology
Plants, Genetically Modified cytology
Plants, Genetically Modified genetics
Plants, Genetically Modified physiology
Sequence Analysis, DNA
Gene Expression Regulation, Plant
Phaseolus physiology
Phosphates metabolism
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2431
- Volume :
- 65
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 24790114
- Full Text :
- https://doi.org/10.1093/jxb/eru183