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Development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste.
- Source :
-
Bioresource technology [Bioresour Technol] 2014 Aug; Vol. 165, pp. 343-9. Date of Electronic Publication: 2014 Mar 22. - Publication Year :
- 2014
-
Abstract
- This work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. Cheese whey was used in Kluyveromyces marxianus and Lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. Subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. The production of ethanol and lactic acid achieved under different conditions was 23gL(-1) and 29gL(-1), respectively. Furthermore, the efficiency of various organic solvents for the esterification reaction was evaluated and toluene was chosen for application in the process. The effect of water content was determined aiming to maximize the product yield and 40mgml(-1) was the optimal enzyme concentration. The bioprocess achieved maximum conversion of 33% constituting a valuable alternative to the application of energy demanding chemically derived methods.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Biofuels
Enzymes, Immobilized
Esterification
Ethanol metabolism
Feasibility Studies
Fungal Proteins
Kluyveromyces metabolism
Lactobacillus metabolism
Liquid-Liquid Extraction
Solutions
Solvents
Temperature
Toluene chemistry
Water chemistry
Biotechnology methods
Dairying
Fermentation
Lactates metabolism
Lipase metabolism
Waste Products
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 165
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 24785788
- Full Text :
- https://doi.org/10.1016/j.biortech.2014.03.053