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Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
- Source :
-
Cellular microbiology [Cell Microbiol] 2014 Oct; Vol. 16 (10), pp. 1503-17. Date of Electronic Publication: 2014 May 23. - Publication Year :
- 2014
-
Abstract
- Uropathogenic Escherichia coli (UPEC) colonizing kidneys is the main cause of acute pyelonephritis. TLR5 that senses flagellin was shown to be highly expressed in the bladder and to participate in host defence against flagellated UPEC, although its role in kidneys still remains elusive. Here we show that TLR5 is expressed in renal medullary collecting duct (MCD) cells, which represent a preferential site of UPEC adhesion. Flagellin, like lipopolysaccharide, stimulated the production of the chemoattractant chemokines CXCL1 and CXCL2, and subsequent migration capacity of neutrophils in cultured wild-type (WT) and Tlr4(-/-) MCDs, but not in Tlr5(-/-) MCDs. UPEC can translocate across intact MCD layers without altering tight junctions. Strikingly, the invasion capacity and transcellular translocation of the UPEC strain HT7 were significantly lower in Tlr5(-/-) than in WT MCDs. The non-motile HT7ΔfliC mutant lacking flagellin also exhibited much lower translocation capacities than the HT7 isolates. Finally, Tlr5(-/-) kidneys exhibited less infiltrating neutrophils than WT kidneys one day after the transurethral inoculation of HT7, and greater delayed renal bacterial loads in the day 4 post-infected Tlr5(-/-) kidneys. Overall, these findings indicate that the epithelial TLR5 participates to renal antibacterial defence, but paradoxically favours the translocation of UPEC across intact MCD cell layers.<br /> (© 2014 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Bacterial Adhesion physiology
Bacterial Load immunology
Chemokine CXCL1 biosynthesis
Chemokine CXCL2 biosynthesis
Escherichia coli Infections microbiology
Female
Kidney Tubules, Collecting cytology
Kidney Tubules, Collecting metabolism
Lipopolysaccharides immunology
Mice
Mice, Inbred C57BL
Neutrophil Infiltration genetics
Neutrophil Infiltration immunology
Neutrophils immunology
Pyelonephritis immunology
Pyelonephritis microbiology
Signal Transduction
Toll-Like Receptor 4 genetics
Toll-Like Receptor 4 immunology
Toll-Like Receptor 5 genetics
Urinary Bladder immunology
Urinary Bladder metabolism
Urinary Tract Infections immunology
Urinary Tract Infections microbiology
Uropathogenic Escherichia coli immunology
Escherichia coli Infections immunology
Flagellin immunology
Kidney Tubules, Collecting immunology
Toll-Like Receptor 5 immunology
Uropathogenic Escherichia coli pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1462-5822
- Volume :
- 16
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cellular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24779433
- Full Text :
- https://doi.org/10.1111/cmi.12306