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Modern analytical supercritical fluid chromatography using columns packed with sub-2 μm particles: a tutorial.

Authors :
Nováková L
Perrenoud AG
Francois I
West C
Lesellier E
Guillarme D
Source :
Analytica chimica acta [Anal Chim Acta] 2014 May 08; Vol. 824, pp. 18-35. Date of Electronic Publication: 2014 Mar 28.
Publication Year :
2014

Abstract

This tutorial provides an overview of the possibilities, limitations and analytical conditions of modern analytical supercritical fluid chromatography (SFC) using columns packed with sub-2 μm particles. In particular, it gives a detailed overview of commercially available modern SFC instrumentation and the detectors that can be employed (UV, MS, ELSD, FID, etc.). Some advice on the choice of the stationary phase dimensions and chemistries, the nature of the mobile phase (choice of organic modifier and additives) and its flow rate as well as the backpressure and temperature are also provided. Finally, several groups of potentially problematic compounds, including lipophilic compounds, hydrophilic substances and basic drugs, are discussed in detail. All these families of analytes can be resolved with SFC but require specific analytical conditions.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-4324
Volume :
824
Database :
MEDLINE
Journal :
Analytica chimica acta
Publication Type :
Academic Journal
Accession number :
24759745
Full Text :
https://doi.org/10.1016/j.aca.2014.03.034