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Involvement of Neisseria meningitidis lipoprotein GNA2091 in the assembly of a subset of outer membrane proteins.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2014 May 30; Vol. 289 (22), pp. 15602-10. Date of Electronic Publication: 2014 Apr 22. - Publication Year :
- 2014
-
Abstract
- GNA2091 of Neisseria meningitidis is a lipoprotein of unknown function that is included in the novel 4CMenB vaccine. Here, we investigated the biological function and the subcellular localization of the protein. We demonstrate that GNA2091 functions in the assembly of outer membrane proteins (OMPs) because its absence resulted in the accumulation of misassembled OMPs. Cell fractionation and protease accessibility experiments showed that the protein is localized at the periplasmic side of the outer membrane. Pulldown experiments revealed that it is not stably associated with the β-barrel assembly machinery, the previously identified complex for OMP assembly. Thus, GNA2091 constitutes a novel outer membrane-based lipoprotein required for OMP assembly. Furthermore, its location at the inner side of the outer membrane indicates that protective immunity elicited by this antigen cannot be due to bactericidal or opsonic activity of antibodies.<br /> (© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Subjects :
- Amino Acid Sequence
Bacterial Outer Membrane Proteins chemistry
Conserved Sequence
Gene Deletion
Lipoproteins genetics
Lipoproteins metabolism
Molecular Sequence Data
Mutagenesis
Neisseria meningitidis, Serogroup B genetics
Phenotype
Porins metabolism
Protein Structure, Tertiary
Bacterial Outer Membrane Proteins metabolism
Bacterial Vaccines metabolism
Neisseria meningitidis, Serogroup B metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 289
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24755216
- Full Text :
- https://doi.org/10.1074/jbc.M113.539510