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New screening method to detect carriage of carbapenemase-producing Enterobacteriaceae in patients within 24 hours.
- Source :
-
The Journal of hospital infection [J Hosp Infect] 2014 May; Vol. 87 (1), pp. 47-9. Date of Electronic Publication: 2014 Mar 26. - Publication Year :
- 2014
-
Abstract
- Rapid identification of patients colonized with carbapenemase-producing Enterobacteriaceae (CPE) is essential to prevent introduction and the spread of CPE in the hospital. This article presents the results of a new screening method to detect patients colonized with CPE within 24h after hospital admission. From high-risk patients rectal and throat swabs were collected and incubated overnight, after which DNA was isolated and tested for the most prevalent CPE genes (KPC, NDM, OXA-48, VIM and IMP) by a ligation-mediated real-time polymerase chain reaction. In 14 months 454 patients were screened; in six patients CPE were detected (carriage rate 1.3%).<br /> (Copyright © 2014 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Aged
Aged, 80 and over
Carrier State diagnosis
Carrier State microbiology
Enterobacteriaceae genetics
Enterobacteriaceae isolation & purification
Enterobacteriaceae Infections microbiology
Humans
Male
Middle Aged
Pharynx microbiology
Rectum microbiology
Time Factors
Bacterial Proteins genetics
Bacteriological Techniques methods
Enterobacteriaceae enzymology
Enterobacteriaceae Infections diagnosis
Mass Screening methods
Molecular Diagnostic Techniques methods
Real-Time Polymerase Chain Reaction methods
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2939
- Volume :
- 87
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of hospital infection
- Publication Type :
- Academic Journal
- Accession number :
- 24746789
- Full Text :
- https://doi.org/10.1016/j.jhin.2014.03.003