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Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology.

Authors :
Satou K
Shiroma A
Teruya K
Shimoji M
Nakano K
Juan A
Tamotsu H
Terabayashi Y
Aoyama M
Teruya M
Suzuki R
Matsuda M
Sekine A
Kinjo N
Kinjo F
Yamaoka Y
Hirano T
Source :
Genome announcements [Genome Announc] 2014 Apr 17; Vol. 2 (2). Date of Electronic Publication: 2014 Apr 17.
Publication Year :
2014

Abstract

We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs.

Details

Language :
English
ISSN :
2169-8287
Volume :
2
Issue :
2
Database :
MEDLINE
Journal :
Genome announcements
Publication Type :
Academic Journal
Accession number :
24744331
Full Text :
https://doi.org/10.1128/genomeA.00286-14