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QTL mapping by pooled-segregant whole-genome sequencing in yeast.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1152, pp. 251-66. - Publication Year :
- 2014
-
Abstract
- Quantitative trait locus (QTL) mapping by pooled-segregant whole-genome sequencing in yeast is a robust methodology for the simultaneous identification of superior genes involved in polygenic traits (e.g., high ethanol tolerance). By crossing two haploid strains with opposite phenotypes, being one of interest, the resulting diploid is sporulated, the meiotic segregants phenotyped, and a pool of selected segregants with the phenotype of interest assembled. The genotyping by pooled-segregant sequencing constitutes a fast and reliable methodology to map all QTL defining the trait of interest. The QTLs can be further analyzed by reciprocal hemizygosity analysis to identify the causative superior alleles that can subsequently be used for yeast strain improvement by targeted genetic engineering.
- Subjects :
- DNA, Fungal genetics
DNA, Fungal isolation & purification
Genome, Fungal genetics
Haploidy
Meiosis genetics
Polymerase Chain Reaction
Propidium metabolism
Spores, Fungal genetics
Yeasts cytology
Yeasts physiology
Chromosome Mapping methods
Genomics methods
Quantitative Trait Loci genetics
Sequence Analysis, DNA methods
Yeasts genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1152
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24744038
- Full Text :
- https://doi.org/10.1007/978-1-4939-0563-8_15