Differential effects of chemoattractants on mast cell recruitment in vivo.

Authors :: de Cássia Campos MR
Toso VD
de Souza DA Jr
Vieira GV
da Silva EZ
Oliver C
Jamur MC
Source :: Cellular immunology [Cell Immunol] 2014 May-Jun; Vol. 289 (1-2), pp. 86-90. Date of Electronic Publication: 2014 Apr 03.
Publication Year :: 2014
Abstract: Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3+rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3+rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3+rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)