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Propofol protects human umbilical vein endothelial cells from cisplatin-induced injury.
- Source :
-
Vascular pharmacology [Vascul Pharmacol] 2014 May-Jun; Vol. 61 (2-3), pp. 72-9. Date of Electronic Publication: 2014 Apr 13. - Publication Year :
- 2014
-
Abstract
- The anticancer drug cisplatin can up-regulate endothelial adhesion molecule expression, and trigger vascular endothelial injury. Propofol, an intravenous anesthetic, can inhibit endothelial adhesion molecule expression in some situations. Here, we explored whether and how propofol improved cisplatin-induced up-regulation of endothelial adhesion molecules in human umbilical vein endothelial cells. Compared with control group, cisplatin reduced endothelial nitric oxide synthase dimer/monomer ratio, activated protein kinase C and enhanced endothelial nitric oxide synthase-Thr495 phosphorylation, decreased nitric oxide production, augmented intercellular adhesion molecule 1 expression and monocyte-endothelial adhesion. These cisplatin-mediated effects were attenuated by propofol treatment. Nω-Nitro-L-arginine methyl ester hydrochloride, a nitric oxide synthase inhibitor, inhibited the effect of propofol on cisplatin-induced intercellular adhesion molecule 1 expression. Propofol improved cisplatin-mediated tetrahydrobiopterin reduction and nitrotyrosine overexpression. Compared with control group, cisplatin and PMA, a protein kinase C activator, both increased endothelial nitric oxide synthase-Thr495 phosphorylation, while propofol and GFX, a protein kinase C inhibitor, both decreased cisplatin-induced endothelial nitric oxide synthase-Thr495 phosphorylation. Our data indicated that propofol, via reducing cisplatin-induced endothelial nitric oxide synthase uncoupling and endothelial nitric oxide synthase-Thr495 phosphorylation, restored nitric oxide production, intercellular adhesion molecule 1 expression and monocyte-endothelial interaction.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Anesthetics, Intravenous pharmacology
Cell Adhesion Molecules drug effects
Cell Adhesion Molecules metabolism
Human Umbilical Vein Endothelial Cells pathology
Humans
Intercellular Adhesion Molecule-1 drug effects
Intercellular Adhesion Molecule-1 metabolism
Monocytes drug effects
Monocytes metabolism
Nitric Oxide metabolism
Nitric Oxide Synthase Type III drug effects
Nitric Oxide Synthase Type III metabolism
Phosphorylation drug effects
Up-Regulation drug effects
Antineoplastic Agents toxicity
Cisplatin toxicity
Human Umbilical Vein Endothelial Cells drug effects
Propofol pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3649
- Volume :
- 61
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Vascular pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 24732179
- Full Text :
- https://doi.org/10.1016/j.vph.2014.04.001