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Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa.
- Source :
-
Enzyme and microbial technology [Enzyme Microb Technol] 2014 May 10; Vol. 58-59, pp. 1-7. Date of Electronic Publication: 2014 Feb 14. - Publication Year :
- 2014
-
Abstract
- Here we describe the identification, purification and characterisation of glycosylated yellow laccase proteins from the basidiomycete fungus Stropharia aeruginosa. Biochemical characterisation of two yellow laccases, Yel1p and Yel3p, show that they are both secreted, monomeric, N-glycosylated proteins of molecular weight around 55kDa with substrate specificities typical of laccases, but lacking the absorption band at 612nm typical of the blue laccase proteins. Low coverage, high throughput 454 transcriptome sequencing in combination with inverse-PCR was used to identify cDNA sequences. One of the cDNA sequences has been assigned to the Yel1p protein on the basis of identity between the translated protein sequence and the peptide data from the purified protein, and the full length gene sequence has been obtained. Biochemical properties, substrate specificities and protein sequence data have been used to discuss the unusual spectroscopic properties of S. aeruginosa proteins in the context of recent theories about the differences between yellow and blue laccases.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Agaricales genetics
Amino Acid Sequence
Base Sequence
Chromatography
Cloning, Molecular
DNA, Complementary genetics
DNA, Fungal genetics
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Fungal Proteins chemistry
Fungal Proteins genetics
Gene Library
Genes, Fungal
Glycoproteins chemistry
Glycoproteins genetics
Glycosylation
Laccase chemistry
Laccase classification
Laccase genetics
Molecular Sequence Data
Molecular Weight
Protein Processing, Post-Translational
RNA, Fungal genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Spectrophotometry, Ultraviolet
Substrate Specificity
Agaricales enzymology
Fungal Proteins isolation & purification
Glycoproteins isolation & purification
Laccase isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0909
- Volume :
- 58-59
- Database :
- MEDLINE
- Journal :
- Enzyme and microbial technology
- Publication Type :
- Academic Journal
- Accession number :
- 24731818
- Full Text :
- https://doi.org/10.1016/j.enzmictec.2014.02.003