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Genetic diversity of feline morbilliviruses isolated in Japan.
- Source :
-
The Journal of general virology [J Gen Virol] 2014 Jul; Vol. 95 (Pt 7), pp. 1464-1468. Date of Electronic Publication: 2014 Apr 11. - Publication Year :
- 2014
-
Abstract
- Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be associated with tubulointerstitial nephritis. Although FmoPV was first described in China in 2012, there has been no report of the isolation of this virus in other countries. In this report, we describe the isolation and characterization of FmoPV from domestic cats in Japan. By using reverse transcription (RT)-PCR, we found that three of 13 urine samples from cats brought to veterinary hospitals were positive for FmoPV. FmoPV strains SS1 to SS3 were isolated from the RT-PCR-positive urine samples. Crandell-Rees feline kidney (CRFK) cells exposed to FmoPV showed cytopathic effects with syncytia formation, and FmoPV N protein was detected by indirect immunofluorescence assays. In addition, pleomorphic virus particles with apparent glycoprotein envelope spikes were observed by electron microscopy. By sequence analysis of FmoPV H and L genes, we found that FmoPVs showed genetic diversity; however, signatures of positive selection were not identified.<br /> (© 2014 The Authors.)
- Subjects :
- Animals
Cats
Cell Line
Cytopathogenic Effect, Viral
Giant Cells virology
Japan
Microscopy, Electron, Transmission
Molecular Sequence Data
Morbillivirus isolation & purification
Morbillivirus Infections virology
Nephritis, Interstitial virology
RNA, Viral genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Urine virology
Virion ultrastructure
Cat Diseases virology
Genetic Variation
Morbillivirus classification
Morbillivirus genetics
Morbillivirus Infections veterinary
Nephritis, Interstitial veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2099
- Volume :
- 95
- Issue :
- Pt 7
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 24728711
- Full Text :
- https://doi.org/10.1099/vir.0.065029-0