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Application of a magnetic molecularly imprinted polymer for the selective extraction and trace detection of lamotrigine in urine and plasma samples.
- Source :
-
Journal of separation science [J Sep Sci] 2014 Jul; Vol. 37 (13), pp. 1610-6. Date of Electronic Publication: 2014 May 02. - Publication Year :
- 2014
-
Abstract
- Magnetic molecularly imprinted polymers have been synthesized for the selective preconcentration and trace determination of lamotrigine (LTG) in urine and plasma samples. The magnetic nanoparticles were modified by tetraethyl orthosilicate and 3-methacryloxypropyl trimethoxysilane before imprinting. The magnetic molecularly imprinted polymers were prepared via surface molecular imprinting technique, using Fe3 O4 as a magnetic component, LTG as template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and 2,2'-azobisisobutyronitrile as a radical initiator in methanol/acetonitrile (50:50, v/v) as the porogen. The obtained sorbent was characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and thermal analysis. Separation of the sorbent from the sample solution was simply achieved by applying an external magnetic field. Determination of the extracted drug was performed by high-performance liquid chromatography with UV detection. Under the optimum extraction conditions, the method detection limits were 0.7 μg/L (based on S/N of 3) for urine samples and 0.5 μg/L for plasma samples. A linear dynamic range of 1-1000 μg/L was obtained for LTF in plasma and urine samples. Finally, the applicability of the proposed method was evaluated by extraction and determination of LTG in urine and plasma samples.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Anticonvulsants blood
Anticonvulsants urine
Humans
Lamotrigine
Molecular Imprinting
Polymers chemical synthesis
Solid Phase Extraction instrumentation
Triazines blood
Triazines urine
Anticonvulsants isolation & purification
Polymers chemistry
Solid Phase Extraction methods
Triazines isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9314
- Volume :
- 37
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of separation science
- Publication Type :
- Academic Journal
- Accession number :
- 24723562
- Full Text :
- https://doi.org/10.1002/jssc.201400188