Back to Search
Start Over
Purification and reconstitution of chloride channels from kidney and trachea.
- Source :
-
Science (New York, N.Y.) [Science] 1989 Jun 23; Vol. 244 (4911), pp. 1469-72. - Publication Year :
- 1989
-
Abstract
- Chloride channels mediate absorption and secretion of fluid in epithelia, and the regulation of these channels is now known to be defective in cystic fibrosis. Indanyl-oxyacetic acid 94 (IAA-94) is a high-affinity ligand for the chloride channel, and an affinity resin based on that structure was developed. Solubilized proteins from kidney and trachea membranes were applied to the affinity matrix, and four proteins with apparent molecular masses of 97, 64, 40, and 27 kilodaltons were eluted from the column by excess IAA-94. A potential-dependent 36Cl- uptake was observed after reconstituting these proteins into liposomes. Three types of chloride channels with single-channel conductances of 26, 100, and 400 picosiemens were observed after fusion of these liposomes with planar lipid bilayers. Similar types of chloride channels have been observed in epithelia.
- Subjects :
- Animals
Bacteriorhodopsins metabolism
Cattle
Chloride Channels
Chlorides physiology
Chlorine metabolism
Chromatography, Affinity
Electric Conductivity
Electrophoresis, Polyacrylamide Gel
Indans
Light
Liposomes metabolism
Membrane Potentials drug effects
Membrane Proteins physiology
Molecular Weight
Radioisotopes
Valinomycin pharmacology
Chlorides isolation & purification
Ion Channels physiology
Kidney Cortex analysis
Membrane Proteins isolation & purification
Trachea analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0036-8075
- Volume :
- 244
- Issue :
- 4911
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 2472007
- Full Text :
- https://doi.org/10.1126/science.2472007