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Comparison of two derivatization methods for the analysis of fatty acids and trans fatty acids in bakery products using gas chromatography.
- Source :
-
TheScientificWorldJournal [ScientificWorldJournal] 2014 Feb 25; Vol. 2014, pp. 906407. Date of Electronic Publication: 2014 Feb 25 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Two different procedures for the methylation of fatty acids (FAs) and trans fatty acids (TFAs) in food fats were compared using gas chromatography (GC-FID). The base-catalyzed followed by an acid-catalyzed method (KOCH3/HCl) and the base-catalyzed followed by (trimethylsilyl)diazomethane (TMS-DM) method were used to prepare FA methyl esters (FAMEs) from lipids extracted from food products. In general, both methods were suitable for the determination of cis/trans FAs. The correlation coefficients (r) between the methods were relatively small (ranging from 0.86 to 0.99) and had a high level of agreement for the most abundant FAs. The significant differences (P = 0.05) can be observed for unsaturated FAs (UFAs), specifically for TFAs. The results from the KOCH3/HCl method showed the lowest recovery values (%R) and higher variation (from 84% to 112%), especially for UFAs. The TMS-DM method had higher R values, less variation (from 90% to 106%), and more balance between variation and %RSD values in intraday and interday measurements (less than 4% and 6%, resp.) than the KOCH3/HCl method, except for C12:0, C14:0, and C18:0. Nevertheless, the KOCH3/HCl method required shorter time and was less expensive than the TMS-DM method which is more convenient for an accurate and thorough analysis of rich cis/trans UFA samples.
Details
- Language :
- English
- ISSN :
- 1537-744X
- Volume :
- 2014
- Database :
- MEDLINE
- Journal :
- TheScientificWorldJournal
- Publication Type :
- Academic Journal
- Accession number :
- 24719581
- Full Text :
- https://doi.org/10.1155/2014/906407