Back to Search
Start Over
Induction of transmissible gastroenteritis coronavirus-neutralizing antibodies in vitro by virus-specific T helper cell hybridomas.
Induction of transmissible gastroenteritis coronavirus-neutralizing antibodies in vitro by virus-specific T helper cell hybridomas.
- Source :
-
The Journal of general virology [J Gen Virol] 1989 Mar; Vol. 70 ( Pt 3), pp. 659-72. - Publication Year :
- 1989
-
Abstract
- Three transmissible gastroenteritis (TGE) virus-specific T helper (Th) cell hybridomas have been generated from virus-primed BALB/c mice, by fusion with the thymoma BW5147. The hybridomas responded to purified u.v.-inactivated TGE virus with interleukin production and growth inhibition. TGE virus recognition by the hybridomas was restricted by the major histocompatibility complex: only splenocytes from syngeneic or semi-syngeneic mice were able to recognize the antigen. The three hybridomas were Thy 1.2+, but did not express detectable levels of Lyt 1 or Lyt 2 antigens by fluorescent cell sorting analysis. Only one hybridoma (T.1J.B5) expressed the L3T4 marker. These hybridomas had helper activity, as they were able to reconstitute in vitro the synthesis of TGE virus-specific antibodies by Th cell-depleted spleen cells from immune BALB/c mice. The antibodies that they induced specifically neutralized by 10(3)- to 10(4)-fold the infectivity of TGE virus, ruling out the possibility of inhibition of virus replication by interferon. These hybridomas could be very useful for identifying antigenic domains in TGE virus recognized by Th cells, which cooperate with B cells in the synthesis of neutralizing antibodies.
- Subjects :
- Animals
Antigenic Variation
Antigens, Surface analysis
Antigens, Viral analysis
B-Lymphocytes immunology
Cell Separation
Epitopes analysis
Mice
Mice, Inbred Strains
Neutralization Tests
Spleen immunology
Antibodies, Viral biosynthesis
Antibody Specificity
Coronaviridae immunology
Hybridomas immunology
T-Lymphocytes, Helper-Inducer immunology
Transmissible gastroenteritis virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 70 ( Pt 3)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 2471795
- Full Text :
- https://doi.org/10.1099/0022-1317-70-3-659