Back to Search
Start Over
WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens.
- Source :
-
Development (Cambridge, England) [Development] 2014 Apr; Vol. 141 (8), pp. 1660-70. - Publication Year :
- 2014
-
Abstract
- Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In the moss Physcomitrella patens, cells at the wound margin of detached leaves become reprogrammed into stem cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like (PpWOX13L) genes, homologs of stem cell regulators in flowering plants, are transiently upregulated and required for the initiation of cell growth during stem cell formation. Concordantly, Δppwox13l deletion mutants fail to upregulate genes encoding homologs of cell wall loosening factors during this process. During the moss life cycle, most of the Δppwox13l mutant zygotes fail to expand and initiate an apical stem cell to form the embryo. Our data show that PpWOX13L genes are required for the initiation of cell growth specifically during stem cell formation, in analogy to WOX stem cell functions in seed plants, but using a different cellular mechanism.
- Subjects :
- Amino Acid Sequence
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Bryopsida growth & development
Cell Proliferation
Cell Wall genetics
Gene Deletion
Gene Expression Regulation, Plant
Meristem cytology
Meristem growth & development
Molecular Sequence Data
Plant Leaves metabolism
Plant Proteins chemistry
Plant Proteins metabolism
Protoplasts metabolism
Regeneration
Stem Cells metabolism
Up-Regulation genetics
Zygote cytology
Zygote growth & development
Bryopsida cytology
Bryopsida genetics
Genes, Plant genetics
Plant Leaves cytology
Plant Proteins genetics
Protoplasts cytology
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 141
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 24715456
- Full Text :
- https://doi.org/10.1242/dev.097444