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18β-glycyrrhetinic acid attenuates anandamide-induced adiposity and high-fat diet induced obesity.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2014 Jul; Vol. 58 (7), pp. 1436-46. Date of Electronic Publication: 2014 Mar 31. - Publication Year :
- 2014
-
Abstract
- Scope: Previous reports suggest that licorice extract has various metabolically beneficial effects and may help to alleviate adiposity and hyperlipidemia. However, underlying anti-obesity mechanisms still remain elusive. Moreover, it is unknown which single ingredient in licorice extract would mediate such effects. We aimed to demonstrate that licorice extract and its active ingredients can inhibit adipocyte differentiation and fat accumulation.<br />Methods and Results: 18β-glycyrrhetinic acid (18β-GA) alleviated the effects of CB1R agonist, anandamide (AEA) on CB1R signaling in a concentration-dependent manner. Consistently, 18β-GA suppressed AEA-induced adipocyte differentiation in 3T3-L1 cells through the downregulation of AEA-induced MAPK activation and expression of adipogenic genes including C/EBP-α and PPAR-γ. The protein levels of fatty acid synthase and stearoyl-CoA desaturase 1 were also decreased and the phosphorylation of acetyl-CoA carboxylase was increased in 18β-GA pretreated cells. The supplementation of 18β-GA significantly lowered body weight, fat weight, and plasma lipids levels in obese animal models.<br />Conclusion: These results may provide a novel insight into the molecular mechanism involved in anti-adipogenic and anti-obesity effects of 18β-GA by suppressing the activation of CB1R induced by AEA. Thus, 18β-GA may exert beneficial effects against obesity-related metabolic disorders.<br /> (© 2014 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- 3T3-L1 Cells
Acetyl-CoA Carboxylase genetics
Acetyl-CoA Carboxylase metabolism
Adipocytes cytology
Adipocytes drug effects
Adiposity
Animals
Blood-Brain Barrier drug effects
Blood-Brain Barrier metabolism
Body Weight drug effects
CCAAT-Enhancer-Binding Protein-alpha genetics
CCAAT-Enhancer-Binding Protein-alpha metabolism
Cell Differentiation drug effects
Glycyrrhetinic Acid pharmacology
Glycyrrhiza chemistry
Lipids blood
Mice
Mice, Inbred C57BL
Obesity chemically induced
PPAR gamma genetics
PPAR gamma metabolism
Phosphorylation
Plant Extracts pharmacology
Stearoyl-CoA Desaturase genetics
Stearoyl-CoA Desaturase metabolism
Arachidonic Acids pharmacology
Diet, High-Fat adverse effects
Endocannabinoids pharmacology
Glycyrrhetinic Acid analogs & derivatives
Obesity metabolism
Polyunsaturated Alkamides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 58
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 24687644
- Full Text :
- https://doi.org/10.1002/mnfr.201300763