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Aging differentially alters the expression of angiogenic genes in a tissue-dependent manner.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 Apr 18; Vol. 446 (4), pp. 1243-9. Date of Electronic Publication: 2014 Mar 28. - Publication Year :
- 2014
-
Abstract
- Organ functions are altered and impaired during aging, thereby resulting in increased morbidity of age-related diseases such as Alzheimer's disease, diabetes, and heart failure in the elderly. Angiogenesis plays a crucial role in the maintenance of tissue homeostasis, and aging is known to reduce the angiogenic capacity in many tissues. Here, we report the differential effects of aging on the expression of angiogenic factors in different tissues, representing a potentially causes for age-related metabolic disorders. PCR-array analysis revealed that many of angiogenic genes were down-regulated in the white adipose tissue (WAT) of aged mice, whereas they were largely up-regulated in the skeletal muscle (SM) of aged mice compared to that in young mice. Consistently, blood vessel density was substantially reduced and hypoxia was exacerbated in WAT of aged mice compared to that in young mice. In contrast, blood vessel density in SM of aged mice was well preserved and was not different from that in young mice. Moreover, we identified that endoplasmic reticulum (ER) stress was strongly induced in both WAT and SM during aging in vivo. We also found that ER stress significantly reduced the expression of angiogenic genes in 3T3-L1 adipocytes, whereas it increased their expression in C2C12 myotubes in vitro. These results collectively indicate that aging differentially affects the expression of angiogenic genes in different tissues, and that aging-associated down-regulation of angiogenic genes in WAT, at least in part through ER stress, is potentially involved in the age-related adipose tissue dysfunction.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- 3T3-L1 Cells
Angiogenesis Inducing Agents metabolism
Animals
Cell Hypoxia
Cell Line
Endoplasmic Reticulum Stress
Mice
Mice, Inbred C57BL
Muscle, Skeletal blood supply
Muscle, Skeletal physiology
Adipose Tissue, White blood supply
Adipose Tissue, White physiology
Aging
Gene Expression Regulation
Neovascularization, Physiologic
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 446
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 24685483
- Full Text :
- https://doi.org/10.1016/j.bbrc.2014.03.098